PRODUCTION OF a-Amylase FROM BANANA PEEL BY Bacillus subtilis
نویسندگان
چکیده
We produced a-amylase from banana peel by Bacillus subtilis through solid state fermentation (SSF). Addition of yeast extract, corn steep liquor, sodium dodesyl sulphate (SDS) and Tween-80 enhanced the a-amylase production under preoptimized fermentation conditions. Maximum enzyme yield (10.25 U/mL) was observed at 35°C after 24 hours of SSF in growth medium containing 0.1% yeast extract, 1% corn steep liquor, 0 15% SOS and 0.1% Tween-80 at pH 7. The crude a-amylase produced under optimum conditions was characterized by studying the effect of pH and temperature on its activity. Activity of a-amylase was maximum (12.18 U/mL) at 60°C and pH 6 which was stable for 3 hours under these conditions.
منابع مشابه
Screening of Various Organic Substrates and the Development of a Suitable Low-Cost Fermentation Medium for the Production of a-Amylase by Bacillus subtilis
The production of extracellular amylase by Bacillus subtilis has been studied in solid-state fermentation (SSF). In a sequential order, various process parameters were optimized for maximum amylase production. The tested process parameters were different solid substrates such as banana husk (BH), water melon husk (WMH), lentil bran (LB), wheat bran (WB), melon husk (MH) and maize oil cake (MOC)...
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